4K34

Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	61.881, 105.032, 72.359
Unit cell angles	90.00, 101.06, 90.00

Refinement procedure

Resolution	42.321 - 2.690
R-factor	0.191
Rwork	0.188
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.092
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.690	2.690
Number of reflections	24838
Completeness [%]	98.5	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	12% PEG3350, 0.1M NaAc, 0.1M Na-Citric (6.5) and 15% 2-3 Butanediol, pH 7.5, VAPOR DIFFUSION, temperature 298K