4K27

Myotonic Dystrophy Type 2 RNA: Structural Studies and Designed Small Molecules that Modulate RNA Function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97856
Spacegroup name	P 41 21 2
Unit cell lengths	75.078, 75.078, 59.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.730 - 2.350
R-factor	0.1961
Rwork	0.194
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.922
Data reduction software	PROCESS
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.900	2.480
High resolution limit [Å]	2.350	2.350
Number of reflections	7540
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	50 mM MES, pH 6.0, 100 mM ammonium acetate, 5 mM Mg sulfate, and 600 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K