4K23
Structure of anti-uPAR Fab ATN-658
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-27 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.313, 132.296, 46.831 |
| Unit cell angles | 90.00, 106.73, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ddq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.080 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.094 | 0.290 |
| Number of reflections | 49312 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 85.7 | 70 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 295 | 20-22% PEG3350, 0.1M Tris(PH 8.0-8.5), vapor diffusion, hanging drop, temperature 295K |
