4K0N

Crystal structure of human CLIC1 C24A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-30
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	42.915, 64.519, 82.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.421 - 1.250
R-factor	0.1487
Rwork	0.148
R-free	0.16960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k0m
RMSD bond length	0.011
RMSD bond angle	1.372
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.920	1.320
High resolution limit [Å]	1.250	1.250
Rmerge	0.071	0.401
Number of reflections	63800
<I/σ(I)>	12.9	3.7
Completeness [%]	99.2	99.4
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.2M Sodium malonate, 20% PEG3350, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K