4K0D
Periplasmic sensor domain of sensor histidine kinase, Adeh_2942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.693, 75.184, 51.031 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 29.882 - 1.997 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25150 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.657 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.360 |
| Number of reflections | 37922 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 89.9 | 62.1 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Wizard II no.11 (10% 2-propanol, 0.1 M sodium cacodylate pH 6.5, 0.2 M zinc acetate), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
