4K03

Crystal structure of Drosophila Cryprochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	71.120, 121.810, 79.720
Unit cell angles	90.00, 114.78, 90.00

Refinement procedure

Resolution	40.486 - 3.200
R-factor	0.1922
Rwork	0.188
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	3.370
High resolution limit [Å]	3.200	3.200
Number of reflections	20372
Completeness [%]	99.7	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM TrisHCl pH 8.0, 17% PEG 3350, 120 mM Mg-Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K