4JZU
Crystal structure of the Bacillus subtilis pyrophosphohydrolase BsRppH bound to a non-hydrolysable triphosphorylated dinucleotide RNA (pcp-pGpG) - first guanosine residue in guanosine binding pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.377, 137.288, 34.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.440 - 1.700 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.20890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF BSRPPH E68A |
RMSD bond length | 0.011 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.760 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.034 | 0.550 |
Number of reflections | 30642 | |
<I/σ(I)> | 23.3 | 2.4 |
Completeness [%] | 96.3 | 90.9 |
Redundancy | 5.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 100 mM Hepes pH 7.5, 25% PEG 1000, vapor diffusion, temperature 293K |