4JZL
Crystal structure of BAP31 vDED at alkaline pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.690, 42.900, 62.670 |
| Unit cell angles | 90.00, 115.87, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.200 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jzp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13838 | |
| <I/σ(I)> | 15.8 | 3.1 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 292 | 100 mM Tris-HCl, 34% PEG2000 monomethyl ether (mme) and 150 mM KBr, pH 8.0, VAPOR DIFFUSION, temperature 292K |
