4JZ9

Carbamate kinase from Giardia lamblia bound to citric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	69.922, 92.740, 101.836
Unit cell angles	90.00, 106.22, 90.00

Refinement procedure

Resolution	46.100 - 2.400
Rwork	0.218
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3KZF AND 2WE4
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.097	0.335
Number of reflections	48667
<I/σ(I)>	7.2	3.3
Completeness [%]	99.4	99.9
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.4 M ammonium citrate dibasic, pH 5.0, 21% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K