4JZ5
High-resolution structure of catalytic domain of endolysin ply40 from bacteriophage P40 of Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 32 |
| Unit cell lengths | 42.332, 42.332, 98.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.661 - 1.100 |
| R-factor | 0.1348 |
| Rwork | 0.134 |
| R-free | 0.14280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hmc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.229 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1063)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.661 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 78926 | |
| Completeness [%] | 98.2 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M Sodium Malonate, 20%(w/v) PEG, pH 7.0 |
