4JYX

Crystal structure of polyprenyl synthase PATL_3739 (TARGET EFI-509195) FROM PSEUDOALTEROMONAS ATLANTICA, COMPLEX WITH INORGANIC PHOSPHATE AND AN UNKNOWN LIGAND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-24
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	73.104, 152.907, 284.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.400 - 2.300
R-factor	0.17896
Rwork	0.177
R-free	0.23882
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jxy
RMSD bond length	0.005
RMSD bond angle	1.044
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.149
Number of reflections	141635
<I/σ(I)>	4.6	1.9
Completeness [%]	99.8	100
Redundancy	8	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2		0.2M LITHIUM SULFATE, 0.1M SODIUM HYDROGEN PHOSPHATE:CITRIC ACID, PH 4.2, 20% PEG1000, VAPOR DIFFUSIO SITTING DROP, TEMPERATURE 298K