4JYK
Structure of E. coli Transcriptional Regulator RutR with bound uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.150, 86.140, 69.019 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 36.750 - 1.700 |
| R-factor | 0.12213 |
| Rwork | 0.120 |
| R-free | 0.16961 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.750 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.690 | |
| Number of reflections | 71325 | |
| Completeness [%] | 91.3 | 36.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 25% PEG 3350, 200 mM Lithium Sulfate, 0.1 M Bis-TRIS pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
