4JY5
Crystal structure of human Fab PGT122, a broadly reactive and potent HIV-1 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.340, 42.040, 45.050 |
Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
Resolution | 38.332 - 1.750 |
R-factor | 0.2004 |
Rwork | 0.198 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jy6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.171 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 38560 | |
<I/σ(I)> | 11.4 | 2.4 |
Completeness [%] | 97.7 | 97.2 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.1 M CAPS, pH 10.5, 0.2 M sodium chloride, 20% w/v PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |