4JXB
RipD (Rv1566c) from Mycobacterium tuberculosis: a non-catalytic NlpC/p60 domain protein, adaptation to peptidoglycan-binding function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.822, 56.114, 57.157 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 56.100 - 1.560 |
| R-factor | 0.17083 |
| Rwork | 0.169 |
| R-free | 0.20406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.110 | 1.640 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.039 | 0.238 |
| Number of reflections | 37671 | |
| <I/σ(I)> | 21 | 3.9 |
| Completeness [%] | 98.4 | 91.4 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 0.1 M Bis-Tris propane/HCl, pH 4.5, 3.5 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
