4JX9
Crystal structure of the complex of peptidyl t-RNA hydrolase from Acinetobacter baumannii with uridine at 1.4A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.945, 65.783, 75.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.040 - 1.400 |
| R-factor | 0.1934 |
| Rwork | 0.193 |
| R-free | 0.20908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iko |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.118 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.040 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 32452 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M HEPES, 25% PEG400, 30% PEG1500, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
