4JWI
Crystal structure of the substrate binding domain of E.coli DnaK in complex with sheep Bac7(35-43)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.914, 161.159, 45.114 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.790 - 1.900 |
| R-factor | 0.2015 |
| Rwork | 0.201 |
| R-free | 0.22920 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3dpo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.790 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.414 |
| Number of reflections | 45783 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 292 | 2.2 M ammonium sulfate, 0.1 M citric acid, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
