4JVZ
Structure of Thermosynechococcus elongatus CcmL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.445, 72.445, 197.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.222 - 2.010 |
| R-factor | 0.2493 |
| Rwork | 0.246 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.839 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.490 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.220 | 0.014 |
| Number of reflections | 35901 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 28.3 | 28.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 0.1 M MES, 1.4 M MgSO4, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
