4JVW
IgM C4-domain from mouse
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.156, 41.207, 67.109 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.19946 |
| Rwork | 0.198 |
| R-free | 0.23415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.354 |
| Number of reflections | 31721 | |
| <I/σ(I)> | 19 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 M HEPES, 15% PEG 8000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
