4JVU
IgM C2-domain from mouse
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.440, 44.780, 54.570 |
| Unit cell angles | 90.00, 118.10, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.1393 |
| Rwork | 0.138 |
| R-free | 0.16814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o0v |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.857 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.038 | 0.309 |
| Number of reflections | 48207 | |
| <I/σ(I)> | 16.1 | 5.2 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 1.0 M LiCl, 0.1 M MES, 23% PEG 6000, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
