4JUN
Crystal structure of H5N1 influenza virus hemagglutinin, clade 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.036, 237.056, 72.857 |
| Unit cell angles | 90.00, 119.18, 90.00 |
Refinement procedure
| Resolution | 49.623 - 2.340 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jum |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
| High resolution limit [Å] | 2.340 | 5.060 | 2.340 |
| Rmerge | 0.089 | 0.040 | 0.302 |
| Number of reflections | 78383 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 87.4 | 96.2 | 55 |
| Redundancy | 2.7 | 3.2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 21% PEG 1500, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
