4JUI
crystal structure of tannase from from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.212, 62.943, 84.412 |
| Unit cell angles | 70.66, 85.49, 79.51 |
Refinement procedure
| Resolution | 19.523 - 1.700 |
| R-factor | 0.1533 |
| Rwork | 0.153 |
| R-free | 0.17960 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 0.305 |
| Number of reflections | 90575 | |
| <I/σ(I)> | 23.3 | 4.9 |
| Completeness [%] | 91.0 | 83.2 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 281 | 20% (w/v) PEG 3000, 0.1M tri-sodium citrate pH 5.5, VAPOR DIFFUSION, temperature 281K |
