4JTM
Structure of the N0 domain of the type II secretin from enterotoxigenic Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 |
| Unit cell lengths | 113.460, 113.460, 24.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.180 - 1.430 |
| R-factor | 0.1869 |
| Rwork | 0.185 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.694 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.180 | 1.470 | |
| High resolution limit [Å] | 1.430 | 6.400 | 1.430 |
| Rmerge | 0.044 | 0.030 | 0.672 |
| Number of reflections | 59059 | 705 | 2759 |
| <I/σ(I)> | 19.09 | 45.59 | 2.07 |
| Completeness [%] | 90.8 | 97.8 | 57.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M TRIS-HCL, 30% PEG2000-MME, pH 8.5, vapor diffusion, sitting drop, temperature 277K |
