4JSQ
Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.770, 300.220, 144.260 |
Unit cell angles | 90.00, 112.86, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.23573 |
Rwork | 0.235 |
R-free | 0.24136 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.823 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 0.581 |
Number of reflections | 257968 | |
<I/σ(I)> | 16.9 | 2.9 |
Completeness [%] | 99.0 | 98.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |