4JS7
Crystal structure of D78N mutant apo form of clavibacter michiganensis expansin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-08-01 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.758, 69.556, 81.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.334 - 2.101 |
R-factor | 0.1548 |
Rwork | 0.152 |
R-free | 0.17650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jjo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.799 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.680 | 2.100 |
Rmerge | 0.038 | 0.064 | |
Number of reflections | 12259 | ||
Completeness [%] | 99.1 | 93.8 | 97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.2M Lithium sulfate, .1M bis-tris, 25%PEG3350 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |