4JQ5

Crystal structure of the human Nup49CCS2+3* coiled-coil segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	63.820, 77.097, 77.197
Unit cell angles	120.04, 90.12, 89.96

Refinement procedure

Resolution	19.830 - 2.195
R-factor	0.2852
Rwork	0.285
R-free	0.29290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.195
Number of reflections	63140

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	10% glycerol, 28% dioxane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K