4JPO
5A resolution structure of Proteasome Assembly Chaperone Hsm3 in complex with a C-terminal fragment of Rpt1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 185.299, 185.299, 357.382 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.870 - 5.000 |
R-factor | 0.2538 |
Rwork | 0.253 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fp7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | SCALA (0.1.29) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 160.474 | 5.590 |
High resolution limit [Å] | 5.000 | 5.000 |
Rmerge | 0.083 | 0.971 |
Number of reflections | 16422 | |
<I/σ(I)> | 20.1 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 19.3 | 19.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 3M Sodium Formate, 100 mM Bis-Tris, 10mM Calcium Chloride, pH 7.0, vapor diffusion, temperature 293K |