4JP8
Crystal structure of Pro-F17H/S324A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-04 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.323, 92.498, 122.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.210 |
| R-factor | 0.2324 |
| Rwork | 0.230 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e1p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.097 | 0.035 | 0.489 |
| Number of reflections | 22172 | ||
| <I/σ(I)> | 37.7 | ||
| Completeness [%] | 99.5 | 97.5 | 99.5 |
| Redundancy | 17.7 | 16 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20mM Tris-HCl pH7.0, 4M Sodium Formate , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
