4JP0
Crystal structure of Cry35Ab1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-11 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.664, 65.128, 117.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.181 |
| R-free | 0.23400 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.526 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.292 |
| Number of reflections | 35100 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 99.3 | 95.3 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 0.72 M NaH2PO4, 0.08 M K2HPO4 and 0.2 M NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
