4JOU
Structural study of Bacillus subtilis HmoB in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 99.597, 99.597, 99.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 2.700 |
| R-factor | 0.19866 |
| Rwork | 0.195 |
| R-free | 0.28038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 293 | 0.5% PEG 2000, 1.3M sodium citrate, 0.1M HEPES pH 7.5, 1mM hemin solution , EVAPORATION, temperature 293K |
