4JOB

Crystal structure of human lysophosphatidic acid phosphatase type 6 complexed with L-(+)-tartrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	44.319, 91.028, 104.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.514 - 2.171
R-factor	0.2048
Rwork	0.201
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.911
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.250
High resolution limit [Å]	2.170	4.670	2.170
Rmerge	0.085	0.056	0.457
Number of reflections	23126
<I/σ(I)>	19.2
Completeness [%]	99.9	99.5	99.3
Redundancy	6.6	6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.2M sodium tartrate dibasic dihydrate, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K