4JO9

Crystal structure of the human Nup49CCS2+3* Nup57CCS3* complex 1:2 stoichiometry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-07
Detector	PSI PILATUS 6M
Wavelength(s)	1.0001
Spacegroup name	P 41 2 2
Unit cell lengths	55.483, 55.483, 191.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.790 - 2.499
R-factor	0.2513
Rwork	0.246
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.499	2.499
Number of reflections	11003
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	3.8	294	0.1 M sodium citrate, pH 3.8, 0.07 M calcium chloride, VAPOR DIFFUSION, temperature 294.0K