4JNO
Crystal structure of Plasmodium falciparum Erythrocyte Binding Antigen 140 (PfEBA-140/BAEBL) Region II in complex with sialyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-07-05 |
Detector | NOIR-1 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.950, 76.030, 81.430 |
Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.2201 |
Rwork | 0.218 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.001 |
RMSD bond angle | 0.437 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.724 | 3.090 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 15336 | |
Completeness [%] | 96.2 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |