4JNG
Schmallenberg virus nucleoprotein-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-01-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.350, 85.960, 77.330 |
| Unit cell angles | 90.00, 102.02, 90.00 |
Refinement procedure
| Resolution | 37.400 - 2.120 |
| R-factor | 0.26548 |
| Rwork | 0.264 |
| R-free | 0.28348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4idx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.303 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.820 | 37.810 |
| High resolution limit [Å] | 2.120 | 2.160 |
| Rmerge | 0.061 | 0.572 |
| Number of reflections | 54303 | |
| <I/σ(I)> | 2 | 2.6 |
| Completeness [%] | 97.9 | 99.2 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 298 | 0.1M Bis-Tris pH5.7, 0.3M NaCl, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
