4JNF
Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97915 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.980, 66.980, 128.759 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.711 - 1.621 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dkx |
RMSD bond length | 0.006 |
RMSD bond angle | 0.844 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Number of reflections | 42873 | |
Completeness [%] | 98.9 | 86.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.0 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |