4JND

Structure of a C.elegans sex determining protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	81.399, 160.093, 77.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.796 - 1.652
R-factor	0.1784
Rwork	0.177
R-free	0.20250
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.240	1.710
High resolution limit [Å]	1.650	2.080	1.650
Rmerge	0.091
Number of reflections	60652
Completeness [%]	99.6	100	98.2
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	28% PEG 400, 0.2M MgCl2, 0.1M HEPES Sodium pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K