4JMK
Structure of dusp8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1. |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.850, 65.850, 124.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.109 - 1.900 |
R-factor | 0.1777 |
Rwork | 0.176 |
R-free | 0.20680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zzw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.119 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 22371 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Hepes, PEG 8000, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 291K |