4JMI

Sec7 domain of ARNO, an exchange factor, at 1.5 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97628
Spacegroup name	P 21 3
Unit cell lengths	89.540, 89.540, 89.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 1.500
R-factor	0.13459
Rwork	0.133
R-free	0.16616
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbv
RMSD bond length	0.007
RMSD bond angle	1.115
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	1.580
High resolution limit [Å]	1.500	1.500
Rmerge		0.342
Number of reflections	38574
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1M MgSO4, Peg 5000 MME 18%, 0.1M Mes pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K