4JLR

Crystal structure of a designed Respiratory Syncytial Virus Immunogen in complex with Motavizumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	149.159, 158.511, 116.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.840 - 2.710
R-factor	0.19424
Rwork	0.191
R-free	0.24926
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.092	0.405
Number of reflections	37515
<I/σ(I)>	19.5	6.8
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	277	20% PEG 1000, 0.1M lithium sulfate, 0.04M phosphate-citrate, 2% glycerol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	4.2	277	20% PEG 1000, 0.1M lithium sulfate, 0.04M phosphate-citrate, 2% glycerol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K