4JLI

Crystal Structure of Escherichia coli Hfq Proximal Pore Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	H 3
Unit cell lengths	104.003, 104.003, 28.185
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.710 - 1.790
R-factor	0.182
Rwork	0.180
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.031
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	21.710
High resolution limit [Å]	1.790
Number of reflections	10698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1