4JKW
Structure of the extracellular domain of butyrophilin BTN3A1 in complex with Isopentenyl pyrophosphate (IPP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96422 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.717, 36.194, 108.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.640 - 2.010 |
| R-factor | 0.23064 |
| Rwork | 0.229 |
| R-free | 0.26838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.640 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.091 | 0.258 |
| Number of reflections | 7571 | |
| <I/σ(I)> | 15.3 | 3.468 |
| Completeness [%] | 92.8 | 80 |
| Redundancy | 5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.12 M ethylene glycols, 0.1 M buffer system 1, 30% PEG 550 MME and PEG 20.000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
