4JJU
Crystal structure of HCV NS5B polymerase in complex with COMPOUND 29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2008-08-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.070, 108.390, 134.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| Rwork | 0.192 |
| R-free | 0.21500 |
| Structure solution method | PDB ENTRY 3MWV |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.085 | 0.356 |
| Number of reflections | 121688 | |
| <I/σ(I)> | 18.03 | 3.86 |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 6.54 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 100 mM MES, 21% PEGmme 5000, 400 mM Ammonium sulfate, 10% glycerol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 262K |
