4JJN

Crystal structure of heterochromatin protein Sir3 in complex with a silenced yeast nucleosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 61
Unit cell lengths	103.682, 103.682, 556.378
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.450 - 3.090
R-factor	0.2325
Rwork	0.231
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER ((CCP4-6.1.1))
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	139.090	139.090	3.260
High resolution limit [Å]	3.090	9.770	3.090
Rmerge	0.039	0.015	0.487
Number of reflections	60025
<I/σ(I)>	14	22.6	1.5
Completeness [%]	97.4	90.4	97.9
Redundancy	2.9	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.05 M sodium cacodylate, 32% 2-methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K