4JJ3
Crystal structure of MamP soaked with iron(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.7374 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.113, 96.078, 54.452 |
| Unit cell angles | 90.00, 113.31, 90.00 |
Refinement procedure
| Resolution | 58.870 - 2.800 |
| R-factor | 0.26576 |
| Rwork | 0.264 |
| R-free | 0.29802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.472 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.191 | |
| Number of reflections | 9413 | |
| Completeness [%] | 98.6 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.2 M ammonium acetate, 20% (w/v) PEG3350 Soaked in Mother Liquor buffered at pH9 and containing 10mM Fe(II)Cl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
