4JJ2
High resolution structure of a C-terminal fragment of the T4 phage gp5 beta-helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.609, 72.764, 130.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.280 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.17770 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELX (2013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.069 | 0.299 |
| Number of reflections | 70372 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.0 | 87 |
| Redundancy | 10.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.03-0.05M CaCl2, 0.1M NaAc pH 5.2, 11-15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
