4JJ0
Crystal structure of MamP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7365, 1.7401, 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.240, 95.810, 54.320 |
| Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
| Resolution | 46.030 - 1.800 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.23945 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.289 |
| Number of reflections | 30227 | |
| Completeness [%] | 87.3 | 85.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M ammonium nitrate and 20% (w/v) PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
