4JIE

Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2012-12-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	68.010, 73.624, 133.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.220 - 2.450
R-factor	0.15546
Rwork	0.153
R-free	0.19540
Structure solution method	Rigid Body Refinement
Starting model (for MR)	2rgl
RMSD bond length	0.010
RMSD bond angle	1.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.820	3.050	2.330
High resolution limit [Å]	2.250	2.830	2.250
Number of reflections	29353
Completeness [%]	77.5	99.9	70.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	288	0.8M K/Na tartrate, 0.1M Na HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 288K