4JI8

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.305, 400.305, 214.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.854 - 3.742
R-factor	0.1393
Rwork	0.137
R-free	0.18730
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.022
RMSD bond angle	2.950
Data scaling software	XSCALE
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.970
High resolution limit [Å]	3.740	10.980	3.740
Rmerge	0.132	0.016	0.016
Number of reflections	177718	7432	27943
<I/σ(I)>	18.11	74.06	1.87
Completeness [%]	99.7	98.6	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K