4JI6

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	401.945, 401.945, 217.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.550
R-factor	0.1535
Rwork	0.151
R-free	0.20100
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.020
RMSD bond angle	2.778
Data scaling software	XSCALE
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.550
Rmerge	0.112
Number of reflections	209634
<I/σ(I)>	9.77
Completeness [%]	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K