4JHK

Crystal structure of Danio rerio SLIP1 in complex with SLBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	70.650, 70.650, 242.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.069 - 2.507
R-factor	0.1948
Rwork	0.192
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.070
High resolution limit [Å]	2.500
Number of reflections	13244
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.17 M K/Na-tartrate, 0.085 M Na-citrate pH 5.6, 1.7 M ammonium sulfate and 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K