4JHJ
Crystal structure of Danio rerio Slip1 in complex with Dbp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 85.185, 85.185, 258.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.770 - 3.250 |
| R-factor | 0.245 |
| Rwork | 0.244 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i2o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.130 | 3.450 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 16724 | |
| <I/σ(I)> | 16.34 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M MES pH 6.0, 18% (w/v) PEG 8000, 0.2 M Ca-Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
